Cancer risk and the complexity of the interactions between environmental and host factors: HENVINET interactive diagrams as simple tools for exploring and understanding the scientific evidence.

BACKGROUND
The development of graphical / visual presentation of the etiology of cancer caused by environmental stress is a process that requires incorporating biological interactions complex between xenobiotics in living and working environment with the gene (gene-environment interactions) and the mechanisms of certain diseases based on genomic and non-genomic in life organism. Traditionally, presentation causal relationships including statistical association between exposure to a xenobiotic and diseases corrected for the effect of potential confounders.


METHOD
In HENVINET FP6 project, we aim to consider the agent together all the known and the mechanisms involved in the development of selected types of cancer. Selection of the type of cancer to the causal diagram based on the corpus of data available and reported relative risk (RR). In establishing a causal diagram complexity of interactions between xenobiotics considered as a priority in the interpretation of the risk of cancer. In addition, gene-environment interactions such as polymorphism gene inserted to repair and Phase I and II enzymes involved in the metabolism of xenobiotics and their elimination. Information about the possibility of age or gender vulnerability is also included.

Diagram user friendly thanks to access to the stage for an information packet and referring to the relevant literature and a glossary of terms. Diagram cover both the chemical and physical agents (ionizing and non-ionizing radiation) and provide basic information about the strength of the relationship between the types of exposures and cancer risk were reported by human studies and is supported by mechanistic studies. Causal diagram HENVINET developed in the project is a valuable source of information for professionals working in the field of environmental health and epidemiology, and as an educational material for students.

Cancer risk and the complexity of the interactions between environmental and host factors: HENVINET interactive diagrams as simple tools for exploring and understanding the scientific evidence.
Cancer risk and the complexity of the interactions between environmental and host factors: HENVINET interactive diagrams as simple tools for exploring and understanding the scientific evidence.


BACKGROUND
Cancer risk results from a complex interaction of environmental exposure with polymorphisms of genes are inherited, genetic load collected during development and capacity non-genomic response to environmental insults. In order to adopt preventive measures are effective and relevant regulatory measures, a comprehensive investigation of the etiology of cancer is very important. Variations and fluctuations in the incidence of cancer in the human population does not reflect the policy of environmental pollution or population distribution of genetic polymorphisms known to be associated with an increased risk of cancer.

Tools that can be used in such comprehensive research, including molecular biology applied to field studies, requires a shift methodological reductionism that has been used until recently as a basic axiom in the interpretation of data. The complexity of interactions between cells, genes and environment, the resonance of living matter with the environment, can be synthesized by biological systems. In the philosophy HENVINET project followed in order to develop interactive causal diagram for cancer investigation with a possible etiology in environmental exposures.

Urea for molecular biology, 99.5%
21113 500 Gms
EUR 6.5
Description: Part A

Xylene for molecular biology, 99.5%
45122 250 ml
EUR 2.81
Description: Part A

Glycine for molecular biology, 99.5%
64072 100 Gms
EUR 1.64
Description: Part A

Acetone for molecular biology, 99.8%
27498 500 ml
EUR 3.76
Description: Part A

Methanol for molecular biology, 99.5%
96446 500 ml
EUR 6.23
Description: Part A

Formamide for molecular biology, 99.5%
30349 500 ml
EUR 5.82
Description: Part A

Imidazole for molecular biology, 99.5%
61510 25 Gms
EUR 5.47
Description: Part A

Chloroform for molecular biology, 99.8%
96764 100 ml
EUR 3.08
Description: Part A

Urea, suitable for molecular biology
GE1210 500g
EUR 53.1

Urea, suitable for molecular biology
GE1210-1 1
EUR 58

Urea, suitable for molecular biology
GE1210-1KG 1 kg
EUR 106.8

Urea, suitable for molecular biology
GE1210-500 500
EUR 33.1

Urea, suitable for molecular biology
GE1210-500G 500 g
EUR 76.8

Triethylamine for molecular biology, 99.5%
55221 500 ml
EUR 4.11
Description: Part A

Ficoll 400® for molecular biology
45460 5 Gms
EUR 15.6
Description: Part B

Agarose UltraPhor for molecular biology
27813 10 Gms
EUR 46.5
Description: Part B

Boric Acid for molecular biology, 99.5%
22311 250 Gms
EUR 4.04
Description: Part A

2-Mercaptoethanol ?For Molecular Biology
MB041-100ML 1 unit
EUR 9.02
Description: 2-Mercaptoethanol ?For Molecular Biology

2-Mercaptoethanol ?For Molecular Biology
MB041-500ML 1 unit
EUR 26.47
Description: 2-Mercaptoethanol ?For Molecular Biology

Triton X-100 for molecular biology
64518 100 ml
EUR 6.02
Description: Part A

MOPS Buffer for molecular biology, 99%
66043 25 Gms
EUR 10.28
Description: Part B

ACES Buffer for molecular biology, 99.5%
27907 5 Gms
EUR 5.4
Description: Part B

Vitamin B12 for molecular biology, 97%
77472 250 Mg
EUR 5.78
Description: Part B

Agarose Low EEO for molecular biology
36601 10 Gms
EUR 5.1
Description: Part B

10X MOPS Buffer for molecular biology
68078 100 ml
EUR 4.5
Description: Part B

CHAPS Buffer for molecular biology, 99.5%
21420 1 Gms
EUR 10.73
Description: Part B

HEPES Buffer for molecular biology, 99.5%
16826 25 Gms
EUR 6.3
Description: Part B

Sodium Azide for molecular biology, 99%
17782 100 Gms
EUR 5.47
Description: Part A

PIPES Buffer for molecular biology, 99%
49159 100 Gms
EUR 26.03
Description: Part B

Agarose High EEO for molecular biology
60645 25 Gms
EUR 13.8
Description: Part B

Jasmonic Acid for molecular biology, 95%
79238 25 Mg
EUR 120.9
Description: Part B

Protamine Sulfate for molecular biology, 90-110%
78349 1 Gms
EUR 22.5
Description: Part B

Oil Red O for molecular biology, 75%
23576 25 Gms
EUR 5.33
Description: Part B

Sodium Chloride for molecular biology, 99.9%
33205 500 Gms
EUR 4.52
Description: Part A

Agarose Medium EEO for molecular biology
10423 10 Gms
EUR 6.75
Description: Part B

Isopropanol (IPA) for molecular biology, 99.8%
38445 100 ml
EUR 1.71
Description: Part A

Acetonitrile (ACN) for molecular biology, 99.9%
62006 250 ml
EUR 4.93
Description: Part A

Ethidium Bromide for molecular biology, 95%
93079 1 Gms
EUR 4.58
Description: Part A

Ammonium Acetate for molecular biology, 98%
37829 100 Gms
EUR 2.12
Description: Part A

Agarose Low Melting for molecular biology
32417 5 Gms
EUR 22.5
Description: Part B

Potassium Acetate for molecular biology, 99.5%
96248 100 Gms
EUR 2.74
Description: Part A

Ammonium Chloride for molecular biology, 99.5%
16992 500 Gms
EUR 4.52
Description: Part A

Ammonium Sulphate for molecular biology, 99.5%
82126 250 Gms
EUR 4.93
Description: Part A

Potassium Chloride for molecular biology, 99.5%
84984 500 Gms
EUR 6.16
Description: Part A

Sodium Bicarbonate for molecular biology, 99.7%
36328 500 Gms
EUR 5.47
Description: Part A

Glycerol (Glycerine) for molecular biology, 99.5%
62417 100 ml
EUR 2.05
Description: Part A

Phenol Crystalline for molecular biology, 99.5%
17286 100 Gms
EUR 3.15
Description: Part A

Phenol, (Carbolic acid) Double distilled for Molecular Biology
PD0252 500g
EUR 192.59

2-Mercaptoethanol for molecular biology, 99%
83759 100 ml
EUR 5.27
Description: Part A

Acriflavine Neutral for molecular biology, 98%
42428 5 Gms
EUR 3.15
Description: Part B

Dichloromethane (DCM) for molecular biology, 99.9%
41512 250 ml
EUR 3.22
Description: Part A

Tetrahydrofuran (THF) for molecular biology, 99.8%
92562 250 ml
EUR 3.28
Description: Part A

Dithioerythritol (DTE) for molecular biology, 99%
53384 1 Gms
EUR 9.53
Description: Part B

Sodium chloride, suitable for molecular biology
GE0307 1kg
EUR 41.33

Sodium chloride, suitable for molecular biology
GE0307-1 1
EUR 45.2

meta-Topolin (mT) for molecular biology, 98%
68913 25 Mg
EUR 17.48
Description: Part B

L-Ascorbic Acid for molecular biology, 99.7%
14116 25 Gms
EUR 1.64
Description: Part A

HEPES Sodium Salt for molecular biology, 99%
32225 25 Gms
EUR 11.85
Description: Part B

Sucrose, GlenBiol, suitable for molecular biology
GC3201-1KG 1 kg
EUR 90

Glycogen ex. Oyster for molecular biology, 85%
49740 1 Gms
EUR 7.5
Description: Part B

D-Sorbitol Powder for molecular biology, 98%
99103 500 Gms
EUR 10.33
Description: Part A

Acetic Acid Glacial for molecular biology, 99.9%
59788 100 ml
EUR 3.56
Description: Part A

Acrylamide 3x cryst. for molecular biology, 99.9%
61346 100 Gms
EUR 5.47
Description: Part A

Brilliant Blue G-250 for molecular biology
64222 5 Gms
EUR 4.65
Description: Part B

Brilliant Blue R-250 for molecular biology
93473 5 Gms
EUR 4.35
Description: Part B

Propidium Iodide (PI) for molecular biology, 95%
11195 10 Mg
EUR 17.63
Description: Part B

Acriflavine Hydrochloride for molecular biology, 98.5%-105%
35188 5 Gms
EUR 4.05
Description: Part B

Polysorbate 20 (Tween 20) for molecular biology
65296 100 Gms
EUR 10.81
Description: Part A

Tris Buffer Superior for molecular biology, 99.9%
37969 100 Gms
EUR 7.59
Description: Part A

Molecular Biology Grade Water for RT-PCR
ML065-1.5ML 1 unit
EUR 7.82
Description: Molecular Biology Grade Water for RT-PCR

10X TE Buffer pH-8.0 for molecular biology
51782 100 ml
EUR 9.45
Description: Part B

10X TBE Buffer pH-8.3 for molecular biology
83170 200 ml
EUR 4.95
Description: Part B

Tris Buffer AR, ACS for molecular biology, 99.9%
71033 100 Gms
EUR 6.02
Description: Part A

10X TAE Buffer pH-8.3 for molecular biology
71989 100 ml
EUR 4.43
Description: Part B

Acrylamide 40% aq. solution for molecular biology
29787 250 ml
EUR 20.53
Description: Part A

MES Monohydrate Buffer for molecular biology, 99%
26854 25 Gms
EUR 11.03
Description: Part B

Glutathione Reduced (GSH) for molecular biology, 99.5%
11514 1 Gms
EUR 5.55
Description: Part B

DL-Dithiothreitol (DTT) for molecular biology, 98%
17315 1 Gms
EUR 5.18
Description: Part B

Trifluoroacetic Acid (TFA) for molecular biology, 99.9%
40801 100 ml
EUR 22.92
Description: Part A

Ammonium Persulphate (APS) for molecular biology, 99%
65553 25 Gms
EUR 1.64
Description: Part A

Sodium Acetate Anhydrous for molecular biology, 99%
22342 250 Gms
EUR 3.56
Description: Part A

Dimethyl Sulphoxide (DMSO) for molecular biology, 99.8%
24075 100 ml
EUR 6.02
Description: Part A

Sodium Acetate Trihydrate for molecular biology, 99.5%
88035 250 Gms
EUR 2.33
Description: Part A

Cesium Sulphate extrapure for molecular biology, 99.9%
57455 10 Gms
EUR 7.5
Description: Part B

Glutathione Oxidized (GSSG) for molecular biology, 99.5%
22151 250 Mg
EUR 22.5
Description: Part B

Guanidine Thiocyanate (GTC) for molecular biology, 99%
80272 100 Gms
EUR 5.47
Description: Part A

Cesium Chloride ultrapure for molecular biology, 99.9%
32355 25 Gms
EUR 18
Description: Part B

Sucrose, GlenBiol™, suitable for molecular biology
GC3201 1kg
EUR 41.25

Sucrose, GlenBiol™, suitable for molecular biology
GC3201-1 1
EUR 45.1

Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve
GS8780 2500ml
EUR 239.63

Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve
GS8780-2500 2500
EUR 249.8

Calcium Chloride Dihydrate for molecular biology, 99.5%
97080 100 Gms
EUR 2.12
Description: Part A

Diethyl Pyrocarbonate (DEPC) for molecular biology, 99%
46791 5 ml
EUR 9
Description: Part B

Pyridine, GlenBiol™, suitable for molecular biology
GS6659 500ml
EUR 230.38

Pyridine, GlenBiol™, suitable for molecular biology
GS6659-2500 2500
EUR 240.3

Pyridine, GlenBiol™, suitable for molecular biology
GS6659-500 500
EUR 95.8

Formamide, GlenBiol™, suitable for molecular biology
GS9663 100ml
EUR 46.83

Formamide, GlenBiol™, suitable for molecular biology
GS9663-100 100
EUR 48.9

20X SSC Buffer pH 6.9-7.1 for molecular biology
12590 200 ml
EUR 2.48
Description: Part B

Bromocresol Purple Sodium Salt for molecular biology
24836 5 Gms
EUR 2.05
Description: Part A

Guanidine Hydrochloride (GHC) for molecular biology, 99.5%
45539 100 Gms
EUR 4.79
Description: Part A

Polyethylene Glycol 8000 (PEG 8000) for molecular biology
54866 500 Gms
EUR 11.97
Description: Part A

10X Tris Buffered Saline (TBS) for molecular biology
83471 500 ml
EUR 14.7
Description: Part B

Magnesium Acetate Tetrahydrate for molecular biology, 99%
50488 100 Gms
EUR 2.74
Description: Part A

Magnesium Chloride Hexahydrate for molecular biology, 99.5%
91417 250 Gms
EUR 4.45
Description: Part A

Magnesium Sulphate Heptahydrate for molecular biology, 99.5%
74440 500 Gms
EUR 6.16
Description: Part A

Tris Hydrochloride (Tris HCl) for molecular biology, 99%
89781 100 Gms
EUR 9.37
Description: Part A

L-Lysine Monohydrate (base) for molecular biology, 99%
45976 25 Gms
EUR 37.63
Description: Part A

Agarose Low EEO Superior Grade for molecular biology
23287 10 Gms
EUR 6
Description: Part B

OORA00229-1L - Molecular Biology Grade UltraPure Water
OORA00229-1L 1L
EUR 149

OORA00230-1L - Molecular Biology Grade UltraPure Water
OORA00230-1L 1L
EUR 279

DTT (Molecular Biology Grade)
CE131 5 g
EUR 44

DTT (Molecular Biology Grade)
CE132 10 g
EUR 88

DTT (Molecular Biology Grade)
CE133 25 g
EUR 170

NAD (Molecular Biology Grade)
CE196 1 g
EUR 34

NAD (Molecular Biology Grade)
CE197 5 g
EUR 137

NBT (Molecular Biology Grade)
CE209 1 g
EUR 96

NBT (Molecular Biology Grade)
CE210 5 g
EUR 345

10X Tris-Glycine-SDS Buffer for molecular biology
57806 200 ml
EUR 3.6
Description: Part B

N,N-Dimethylformamide (DMF) for molecular biology, 99.9%
24017 100 ml
EUR 3.08
Description: Part A

Cesium Chloride extrapure AR for molecular biology, 99.9%
22966 25 Gms
EUR 14.63
Description: Part B

10X Tris-Tricine-SDS Buffer for molecular biology
37852 500 ml
EUR 9.08
Description: Part B

EDTA Disodium Salt Dihydrate for molecular biology, 99.5%
43272 100 Gms
EUR 2.26
Description: Part A

DMSO, Molecular Biology Grade
40470006-1 100 mL
EUR 108.14

DMSO, Molecular Biology Grade
40470006-2 250 mL
EUR 182.67

DMSO, Molecular Biology Grade
40470006-3 500 mL
EUR 342.49

EGTA, Molecular Biology Grade
40500028-2 50 g
EUR 130.53

EGTA, Molecular Biology Grade
40500028-3 100 g
EUR 217.78

EGTA, Molecular Biology Grade
40500028-4 500 g
EUR 734.55

EGTA, Molecular Biology Grade
40500028-5 1 kg
EUR 1119.67

EGTA, Molecular Biology Grade
40500028-6 2 kg
EUR 2070.09

BCIP (Molecular Biology Grade)
CE108 250 mg Ask for price

BCIP (Molecular Biology Grade)
CE109 1 g
EUR 82

DAPI (Molecular Biology Grade)
CE117 5 mg
EUR 40

DAPI (Molecular Biology Grade)
CE118 25 mg
EUR 133

DAPI (Molecular Biology Grade)
CE119 100 mg
EUR 281

Tris (Molecular Biology Grade)
CE237 500 g
EUR 79

Tris (Molecular Biology Grade)
CE238 1 kg
EUR 135

Tris (Molecular Biology Grade)
CE239 5 kg
EUR 608

Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve
GS3406 2500ml
EUR 111.53

Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve
GS3406-2500 2500
EUR 116.2

Chloroform : Isoamyl Alcohol (24:1) for molecular biology
85563 100 ml
EUR 8.48
Description: Part B

1X Phosphate Buffered Saline (PBS) for molecular biology
95131 100 ml
EUR 3.98
Description: Part B

Saponin ex. Quillaja for molecular biology, 9% Sapogenin
80967 25 Gms
EUR 45
Description: Part B

CHAPS (Molecular Biology Grade)
CE114 1 g Ask for price

CHAPS (Molecular Biology Grade)
CE115 5 g
EUR 97

CHAPS (Molecular Biology Grade)
CE116 25 g
EUR 319

HEPES (Molecular Biology Grade)
CE171 100 g
EUR 40

HEPES (Molecular Biology Grade)
CE172 500 g
EUR 162

HEPES (Molecular Biology Grade)
CE173 1 kg
EUR 285

Water (Molecular Biology Grade)
CE243 500 ml
EUR 62.4

Water (Molecular Biology Grade)
CE244 1 l
EUR 35

Molecular Biology Grade Water
ML024-100ML 1 unit
EUR 3.54
Description: Molecular Biology Grade Water

Molecular Biology Grade Water
ML024-10X100ML 1 unit
EUR 29.52
Description: Molecular Biology Grade Water

Molecular Biology Grade Water
ML024-10X500ML 1 unit
EUR 87.91
Description: Molecular Biology Grade Water

Molecular Biology Grade Water
ML024-500ML 1 unit
EUR 12.56
Description: Molecular Biology Grade Water

Molecular Biology Grade Water
ML064-100ML 1 unit
EUR 3.67
Description: Molecular Biology Grade Water

Molecular Biology Grade Water
ML064-10X100ML 1 unit
EUR 25.38
Description: Molecular Biology Grade Water

Molecular Biology Grade Water
ML064-500ML 1 unit
EUR 10.81
Description: Molecular Biology Grade Water

10X Phosphate Buffered Saline (PBS) for molecular biology
78529 100 ml
EUR 4.95
Description: Part B

Agarose, low EEO, GlenBiol, suitable for molecular biology
GE6258-100G 100 g
EUR 217.2

Potassium Dihydrogen Orthophosphate for molecular biology, 99.5%
54358 250 Gms
EUR 4.11
Description: Part A

Dimethylformamide, GlenBiol™, suitable for molecular biology
GS6580 2500ml
EUR 102.9

Dimethylformamide, GlenBiol™, suitable for molecular biology
GS6580-2500 2500
EUR 107.3

Glycin (Molecular Biology Grade)
CE158 1 kg
EUR 61

Glycin (Molecular Biology Grade)
CE159 5 kg
EUR 221

DEAE Cellulose 23 analytical grade for molecular biology
57136 5 Gms
EUR 20.7
Description: Part B

DEAE Cellulose 32 analytical grade for molecular biology
92321 5 Gms
EUR 25.73
Description: Part B

DEAE Cellulose 52 analytical grade for molecular biology
10529 5 Gms
EUR 16.28
Description: Part B

DEAE Cellulose 11 analytical grade for molecular biology
47478 5 Gms
EUR 18.38
Description: Part B

EDTA Tetrasodium Salt Dihydrate for molecular biology, 99.5%
45247 100 Gms
EUR 6.16
Description: Part A

OORA00218-100IU - DNA Ligase T4 Molecular Biology Grade
OORA00218-100IU 100Units
EUR 129

20X SSPE Buffer pH-7.4 suitable for molecular biology
78305 200 ml
EUR 6.75
Description: Part B

HEPES Buffer 1M Solution pH-7.3 for molecular biology
78805 100 ml
EUR 25.2
Description: Part B

Agarose, Molecular Biology Grade
40100164-1 25 g Ask for price

Agarose, Molecular Biology Grade
40100164-2 50 g Ask for price

Agarose, Molecular Biology Grade
40100164-3 100 g Ask for price

Agarose, Molecular Biology Grade
40100164-4 500 g Ask for price

Agarose, Molecular Biology Grade
40100164-5 1 kg Ask for price

Agarose (Molecular Biology Grade)
abx299715-100g 100 µg Ask for price

Agarose (Molecular Biology Grade)
abx299715-20g 20 µg
EUR 525

Agarose (Molecular Biology Grade)
abx299715-50g 50 µg Ask for price

5-Fluoroorotic Acid (5-FOA) for molecular biology, 98%
78510 250 Mg
EUR 29.78
Description: Part B

Lysozyme (Molecular Biology Grade)
CE188 1 g
EUR 70.8

Lysozyme (Molecular Biology Grade)
CE189 10 g
EUR 80

Lysozyme (Molecular Biology Grade)
CE189L 50 g
EUR 310

Lysozyme (Molecular Biology Grade)
CE189XL 250 g
EUR 1050

Sodium Citrate Tribasic Dihydrate for molecular biology, 99%
67331 250 Gms
EUR 4.38
Description: Part A

Water, distilled, GlenBiol™, suitable for molecular biology
GK8512 1l
EUR 55.02


RESULTS
causal diagram representing knowledge and tools integrated seed for their future development and the development of a similar diagram for other environment-related diseases such as asthma or infertility. In this paper the development and implementation of a causal diagram for cancer are presented and discussed.