Cancer risk and the complexity of the interactions between environmental and host factors: HENVINET interactive diagrams as simple tools for exploring and understanding the scientific evidence.

BACKGROUND
The development of graphical / visual presentation of the etiology of cancer caused by environmental stress is a process that requires incorporating biological interactions complex between xenobiotics in living and working environment with the gene (gene-environment interactions) and the mechanisms of certain diseases based on genomic and non-genomic in life organism. Traditionally, presentation causal relationships including statistical association between exposure to a xenobiotic and diseases corrected for the effect of potential confounders.


METHOD
In HENVINET FP6 project, we aim to consider the agent together all the known and the mechanisms involved in the development of selected types of cancer. Selection of the type of cancer to the causal diagram based on the corpus of data available and reported relative risk (RR). In establishing a causal diagram complexity of interactions between xenobiotics considered as a priority in the interpretation of the risk of cancer. In addition, gene-environment interactions such as polymorphism gene inserted to repair and Phase I and II enzymes involved in the metabolism of xenobiotics and their elimination. Information about the possibility of age or gender vulnerability is also included.

Diagram user friendly thanks to access to the stage for an information packet and referring to the relevant literature and a glossary of terms. Diagram cover both the chemical and physical agents (ionizing and non-ionizing radiation) and provide basic information about the strength of the relationship between the types of exposures and cancer risk were reported by human studies and is supported by mechanistic studies. Causal diagram HENVINET developed in the project is a valuable source of information for professionals working in the field of environmental health and epidemiology, and as an educational material for students.

 Cancer risk and the complexity of the interactions between environmental and host factors: HENVINET interactive diagrams as simple tools for exploring and understanding the scientific evidence.
Cancer risk and the complexity of the interactions between environmental and host factors: HENVINET interactive diagrams as simple tools for exploring and understanding the scientific evidence.


BACKGROUND
Cancer risk results from a complex interaction of environmental exposure with polymorphisms of genes are inherited, genetic load collected during development and capacity non-genomic response to environmental insults. In order to adopt preventive measures are effective and relevant regulatory measures, a comprehensive investigation of the etiology of cancer is very important. Variations and fluctuations in the incidence of cancer in the human population does not reflect the policy of environmental pollution or population distribution of genetic polymorphisms known to be associated with an increased risk of cancer.

Tools that can be used in such comprehensive research, including molecular biology applied to field studies, requires a shift methodological reductionism that has been used until recently as a basic axiom in the interpretation of data. The complexity of interactions between cells, genes and environment, the resonance of living matter with the environment, can be synthesized by biological systems. In the philosophy HENVINET project followed in order to develop interactive causal diagram for cancer investigation with a possible etiology in environmental exposures.

Phenol, (Carbolic acid) Double distilled for Molecular Biology

PD0252 500g
EUR 192.59

Sodium chloride, suitable for molecular biology

GE0307-1 1
EUR 45.2

Sucrose, GlenBiol, suitable for molecular biology

GC3201-1KG 1 kg
EUR 90

NAD (Molecular Biology Grade)

CE196 1 g
EUR 72

NAD (Molecular Biology Grade)

CE197 5 g
EUR 165.6

NBT (Molecular Biology Grade)

CE209 1 g
EUR 123.6

NBT (Molecular Biology Grade)

CE210 5 g
EUR 360

DTT (Molecular Biology Grade)

CE131 5 g
EUR 93.6

DTT (Molecular Biology Grade)

CE132 10 g
EUR 133.2

DTT (Molecular Biology Grade)

CE133 25 g
EUR 243.6

Tris (Molecular Biology Grade)

CE237 500 g
EUR 106.8

Tris (Molecular Biology Grade)

CE238 1 kg
EUR 153.6

Tris (Molecular Biology Grade)

CE239 5 kg
EUR 535.2

BCIP (Molecular Biology Grade)

CE108 250 mg
EUR 75.6

BCIP (Molecular Biology Grade)

CE109 1 g
EUR 108

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 382.8

100mL Molecular Biology Grade

46-000-CI PK6
EUR 74.4

500mL Molecular Biology Grade

46-000-CV PK6
EUR 138

HEPES (Molecular Biology Grade)

CE171 100 g
EUR 98.4

HEPES (Molecular Biology Grade)

CE172 500 g
EUR 268.8

HEPES (Molecular Biology Grade)

CE173 1 kg
EUR 424.8

Water (Molecular Biology Grade)

CE243 500 ml
EUR 62.4

Water (Molecular Biology Grade)

CE244 1 l
EUR 67.2

CHAPS (Molecular Biology Grade)

CE114 1 g
EUR 66

CHAPS (Molecular Biology Grade)

CE115 5 g
EUR 157.2

CHAPS (Molecular Biology Grade)

CE116 25 g
EUR 492

Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve

GS8780-2500 2500
EUR 249.8

Tween20 (Molecular Biology Grade)

CE242 1 l
EUR 106.8

Glycine (Molecular Biology Grade)

CE158 1 kg
EUR 84

Glycine (Molecular Biology Grade)

CE159 5 kg
EUR 228

Sucrose, GlenBiol™, suitable for molecular biology

GC3201-1 1
EUR 45.1

Lysozyme (Molecular Biology Grade)

CE188 1 g
EUR 70.8

Lysozyme (Molecular Biology Grade)

CE189 10 g
EUR 247.2

Pyridine, GlenBiol™, suitable for molecular biology

GS6659-2500 2500
EUR 240.3

Pyridine, GlenBiol™, suitable for molecular biology

GS6659-500 500
EUR 95.8

Formamide, GlenBiol™, suitable for molecular biology

GS9663-100 100
EUR 48.9

1L Molecular Biology Grade Water

46-000-CM PK6
EUR 196.8

Tween 20, Molecular Biology Grade

T9100-010 100ml
EUR 86.4

Tween 20, Molecular Biology Grade

T9100-050 500ml
EUR 133.2

Tween 20, Molecular Biology Grade

T9100-100 1L
EUR 160.8

D(+)-Sucrose (Molecular Biology Grade)

CE224 500 g
EUR 67.2

D(+)-Sucrose (Molecular Biology Grade)

CE225 1 kg
EUR 84

D(+)-Sucrose (Molecular Biology Grade)

CE226 5 kg
EUR 207.6

Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve

GS3406-2500 2500
EUR 116.2

MOPS buffer (Molecular Biology Grade)

CE194 100 g
EUR 102

MOPS buffer (Molecular Biology Grade)

CE195 250 g
EUR 169.2

Glycerol 87 % (Molecular Biology Grade)

CE154 1 l
EUR 93.6

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 217.2

Formamide deionized (Molecular Biology Grade)

CE145 500 ml
EUR 87.6

Formamide deionized (Molecular Biology Grade)

CE146 1 l
EUR 120

TritonX-100 (Molecular Biology Grade)

CE240 500 ml
EUR 67.2

TritonX-100 (Molecular Biology Grade)

CE241 1 l
EUR 79.2

Dimethylsulfoxide (Molecular Biology Grade)

CE120 100 ml
EUR 66

Dimethylsulfoxide (Molecular Biology Grade)

CE121 500 ml
EUR 110.4

Dimethylformamide, GlenBiol™, suitable for molecular biology

GS6580-2500 2500
EUR 107.3

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1L 1 l
EUR 92.4

Acetonitrile 50, GlenBiol™, suitable for molecular biology

GS0247-1 1
EUR 40.7

Acetonitrile 50, GlenBiol™, suitable for molecular biology

GS0247-2500 2500
EUR 61.3

Acetonitrile 10, GlenBiol™, suitable for molecular biology

GS0969-1 1
EUR 47

Acetonitrile 10, GlenBiol™, suitable for molecular biology

GS0969-2500 2500
EUR 73.9

Acetonitrile 30, GlenBiol™, suitable for molecular biology

GS8649-1 1
EUR 43.1

Acetonitrile 30, GlenBiol™, suitable for molecular biology

GS8649-2500 2500
EUR 66.5

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1 1
EUR 60.1

Water, Ultrapure Molecular Biology Grade

41024-4L 4L
EUR 145.2
Description: Minimum order quantity: 1 unit of 4L

Sodium chloride (Molecular Biology Grade)

CE205 500 g
EUR 62.4

Sodium chloride (Molecular Biology Grade)

CE206 1 kg
EUR 70.8

Sodium chloride (Molecular Biology Grade)

CE207 5 kg
EUR 123.6

Bis-Acrylamid (Molecular Biology Grade)

CE110 50 g
EUR 94.8

Bis-Acrylamid (Molecular Biology Grade)

CE111 250 g
EUR 259.2

Ammonia solution, GlenBiol™, suitable for molecular biology

GS8853-100 100
EUR 91.2

Urea Crystalline (Molecular Biology Grade)

CE167 1 kg
EUR 72

Urea Crystalline (Molecular Biology Grade)

CE168 5 kg
EUR 181.2

Ammonium sulfate (Molecular Biology Grade)

CE105 250 g
EUR 55.2

Ammonium sulfate (Molecular Biology Grade)

CE106 1 kg
EUR 72

Ammonium sulfate (Molecular Biology Grade)

CE107 5 kg
EUR 153.6

Agarose, low EEO, GlenBiol™, suitable for molecular biology

GE6258-100 100
EUR 150.4

Agarose, low EEO, GlenBiol™, suitable for molecular biology

GE6258-25 25
EUR 60.1

SSC Buffer (20X) (Molecular Biology Grade)

CE229 1 l
EUR 86.4

XTT sodium salt (Molecular Biology Grade)

CE250 100 mg
EUR 208.8

XTT sodium salt (Molecular Biology Grade)

CE251 500 mg
EUR 612

Tris - Hydrochloride (Molecular Biology Grade)

CE234 250 g
EUR 99.6

Tris - Hydrochloride (Molecular Biology Grade)

CE235 500 g
EUR 144

Tris - Hydrochloride (Molecular Biology Grade)

CE236 1 kg
EUR 223.2

Glycerol waterfree (Molecular Biology Grade)

CE155 500 ml
EUR 78

Glycerol waterfree (Molecular Biology Grade)

CE156 1 l
EUR 102

Glycerol waterfree (Molecular Biology Grade)

CE157 2.5 l
EUR 170.4

NADP - sodium salt (Molecular Biology Grade)

CE200 250 mg
EUR 92.4

NADP - sodium salt (Molecular Biology Grade)

CE201 1 g
EUR 190.8

Guanidine Thiocyanate (Molecular Biology Grade)

CE164 100 g
EUR 86.4

Guanidine Thiocyanate (Molecular Biology Grade)

CE165 500 g
EUR 192

Guanidine Thiocyanate (Molecular Biology Grade)

CE166 1 kg
EUR 307.2

NADH - Disodium salt (Molecular Biology Grade)

CE198 1 g
EUR 91.2

NADH - Disodium salt (Molecular Biology Grade)

CE199 5 g
EUR 244.8

Guanidine - Hydrochloride (Molecular Biology Grade)

CE163 1 kg
EUR 352.8

Guanidine - Hydrochloride (Molecular Biology Grade)

CE160 100 g
EUR 93.6

Guanidine - Hydrochloride (Molecular Biology Grade)

CE161 250 g
EUR 153.6

Guanidine - Hydrochloride (Molecular Biology Grade)

CE162 500 g
EUR 232.8

Yeast extract powder (Molecular Biology Grade)

CE169 500 g
EUR 133.2

D(+)-Glucose waterfree (Molecular Biology Grade)

CE148 500 g
EUR 67.2

D(+)-Glucose waterfree (Molecular Biology Grade)

CE149 1 kg
EUR 75.6

D(+)-Glucose waterfree (Molecular Biology Grade)

CE150 5 kg
EUR 180

Hyaluronidase Grade I (Molecular Biology Grade)

CE174 1 g
EUR 232.8

Hyaluronidase Grade I (Molecular Biology Grade)

CE175 5 g
EUR 920.4

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE202 25 mg
EUR 70.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE203 100 mg
EUR 126

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE204 500 mg
EUR 374.4

3-Methylbutan-1-ol, GlenBiol™, suitable for molecular biology

GS6015-100 100
EUR 74.5

3-Methylbutan-1-ol, GlenBiol™, suitable for molecular biology

GS6015-500 500
EUR 301.4

TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology

GE6710-1 1
EUR 67.9

TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology

GE6710-250 250
EUR 22.7

Di-iso-propylethylamine, GlenBiol™, suitable for molecular biology

GS7181-100 100
EUR 143.3

Di-iso-propylethylamine, GlenBiol™, suitable for molecular biology

GS7181-500 500
EUR 474.2

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE100 50 g
EUR 128.4

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE101 100 g
EUR 193.2

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE102 250 g
EUR 387.6

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE103 500 g
EUR 656.4

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE104 1 kg
EUR 1162.8

Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0)

GX7489-1 1
EUR 22.7

Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0)

GX7489-2500 2500
EUR 45.2

Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0)

GX7489-500 500
EUR 15.9

Water, Ultra Pure, Sterile Molecular Biology Grade

18-193 100ml/Unit
EUR 268

Water, Ultra Pure, Sterile Molecular Biology Grade

18-194 500ml/Unit
EUR 294

Water, Ultra Pure, Sterile Molecular Biology Grade

18-195 1000ml/Unit
EUR 308

Water, Ultra Pure, Sterile Molecular Biology Grade

18-196 4 x 1L/Unit
EUR 471

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE135 250 g
EUR 72

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE136 500 g
EUR 86.4

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE137 1 kg
EUR 124.8

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE138 5 kg
EUR 418.8

Ammonium acetate 7.5M solution, GlenBiol™, suitable for molecular biology

GX5459-1 1
EUR 271.2

Ammonium acetate 7.5M solution, GlenBiol™, suitable for molecular biology

GX5459-250 250
EUR 90.4

N-Methyl-2-pyrrolidone, GlenBiol™, suitable for molecular biology

GS5724-2500 2500
EUR 126.3

Ammonium acetate 5M solution, GlenBiol™, suitable for molecular biology

GX2678-1 1
EUR 271.2

Ammonium acetate 5M solution, GlenBiol™, suitable for molecular biology

GX2678-250 250
EUR 90.4

Magnesium acetate - Tetrahydrate (Molecular Biology Grade)

CE190 500 g
EUR 98.4

EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade)

CE141 500 ml
EUR 87.6

TRIS-Glycine-SDS Buffer 10X, GlenBiol™, suitable for molecular biology

GE9118-1 1
EUR 75.3

TRIS-Glycine-SDS Buffer 10X, GlenBiol™, suitable for molecular biology

GE9118-250 250
EUR 30.1

Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g

41028-100G 100G
EUR 266.4
Description: Minimum order quantity: 1 unit of 100G

Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g

41028-25G 25G
EUR 130.8
Description: Minimum order quantity: 1 unit of 25G

Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g

41028-500G 500G
EUR 862.8
Description: Minimum order quantity: 1 unit of 500G

Dichloromethane, GlenBiol™, suitable for molecular biology stabilised with amylene

GS0032-1 1
EUR 72.3

Dichloromethane, GlenBiol™, suitable for molecular biology stabilised with amylene

GS0032-2500 2500
EUR 139.6

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 201.6
Description: Bioscience Mol Bio; Agarose

Bulk Beads Zr 0.1mm Triple-Pure Molecular Biology Grade 250g

HOM3048 250G
EUR 637.2

Bulk Beads Zr 0.5mm Triple-Pure Molecular Biology Grade 250g

HOM3050 250G
EUR 637.2

Bulk Beads Zr 1.0mm Triple-Pure Molecular Biology Grade 250g

HOM3052 250G
EUR 637.2

Bulk Beads Zr 1.5mm Triple-Pure Molecular Biology Grade 250g

HOM3054 250G
EUR 637.2

Bulk Beads Zr 3.0mm Triple-Pure Molecular Biology Grade 300g

HOM3056 300G
EUR 637.2

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE178 500 g
EUR 109.2

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE179 2.5 kg
EUR 301.2

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE180 500 g
EUR 104.4

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE181 2.5 kg
EUR 295.2

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE182 500 g
EUR 108

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE183 2.5 kg
EUR 301.2

LB-Medium - Powder according to Miller (Molecular Biology Grade)

CE184 2.5 kg
EUR 295.2

Bulk Beads, Zirconium, 0.1mm, Triple-Pure Molecular Biology Grade, 250g

D1132-01TP 1 PC
EUR 482.6

Bulk Beads, Zirconium, 0.5mm, Triple-Pure Molecular Biology Grade, 250g

D1132-05TP 1 PC
EUR 482.6

Bulk Beads, Zirconium, 1.0mm, Triple-Pure Molecular Biology Grade, 250g

D1132-10TP 1 PC
EUR 482.6

Bulk Beads, Zirconium, 1.5mm, Triple-Pure Molecular Biology Grade, 250g

D1132-15TP 1 PC
EUR 482.6

Bulk Beads, Zirconium, 3.0mm, Triple-Pure Molecular Biology Grade, 300g

D1132-30TP 1 PC
EUR 482.6

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3669.6
Description: Bioscience Mol Bio; Agarose

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 926.4
Description: Bioscience Mol Bio; Agarose

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2445.6
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 4484.4
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2490
Description: Bioscience Mol Bio; Agarose

Malachite green carbinol base

GT7477-100G 100 g
EUR 115.2

Malachite green carbinol base

GT7477-100 100
EUR 66

Malachite Green carbinol hydrochloride

GT8088-100G 100 g
EUR 136.8

Malachite Green carbinol hydrochloride

GT8088-25G 25 g
EUR 64.8

Malachite Green carbinol hydrochloride

GT8088-100 100
EUR 83.1

Malachite Green carbinol hydrochloride

GT8088-25 25
EUR 23.8

30UL CLEAR PIPET TIPS FOR MOLECULAR DEVICE LIBERTY.

LT-384-R 3840/pk
EUR 2631.6
Description: Robotic Tips; Robotic tips - Axygen

30ul Clear Pipet Tips for Molecular Device Liberty.

LT384R PK19200
EUR 4483.2

30uL Black Tips For Molecular Device Liberty N/ST

PIP1678 PK19200
EUR 4606.8

Molecular sieves 200g

TIT1072 200G
EUR 711.6

Molecular Weight Marker

abx098957-15ul 15 ul
EUR 126

Agarose, Molecular Grade

BIO-41025 500g Ask for price

Agarose, Molecular Grade

BIO-41026 100g Ask for price

5-20% 12-well PAGEL 10/pk for low molecular weight

2331120 1unit
EUR 452.4

ELISA kit for Human LMWK (Low Molecular Weight Kininogen)

ELK1799 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Low Molecular Weight Kininogen from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse HMWK (High Molecular Weight Kininogen)

ELK7788 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of High Molecular Weight Kininogen from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human HMWK (High Molecular Weight Kininogen)

ELK2201 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of High Molecular Weight Kininogen from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

15%S 12-well c-PAGEL 10/pk for low molecular weight

2331691 1unit
EUR 357.6
Description: 1,000-100,000/ -

15% S 18-well e-PAGEL 10/pk for low molecular weight

2331760 1unit
EUR 355.2
Description: 1,000-100,000/ -

15% S 14-well e-PAGEL 10/pk for low molecular weight

2331860 1unit
EUR 355.2
Description: 1,000-100,000/ -

ELISA kit for Rat LOW Molecular Weight Adiponectin (LOW-ADP)

KTE101014-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Rat LOW Molecular Weight Adiponectin (LOW-ADP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat LOW Molecular Weight Adiponectin (LOW-ADP)

KTE101014-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Rat LOW Molecular Weight Adiponectin (LOW-ADP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.


RESULTS
causal diagram representing knowledge and tools integrated seed for their future development and the development of a similar diagram for other environment-related diseases such as asthma or infertility. In this paper the development and implementation of a causal diagram for cancer are presented and discussed.