A Plant Biologist’s Toolbox to Study Translation.

In a variety of species and biological questions, more and more studies that combine data translation to better assess how gene regulation occurs at a rate of protein synthesis. The inclusion of data translation improves upon, and has proven to be more accurate than, studies transcription course. However, there are many different techniques available for measuring the translation and can be difficult, especially for young scientists or aspire, to determine the best method is applied in particular situations. We have put together this review in order to enhance understanding and promote the use of translation in plant biology.

Our methods include a variety of methods to measure changes in the global translation (eg, radiolabeling, polysome profiles, or puromycylation), translation of a single gene (eg, fluorescent reporter construction, toeprinting, or mapping the density of the ribosome), sequencing-based to uncover the whole translatome (for example, Ribo-seq or translating ribosome affinity purification), and mass spectrometry-based method for identifying changes in the proteome (eg, stable isotope labeling with amino acids in cell culture or bioorthogonal marking noncanonical amino acids).

The benefits and limitations of each method are discussed with special note of how the application of other model systems may be extended for use in plants. In order to make this emerging field more accessible to students and scientists are newer, our review includes a comprehensive glossary defining key terms.

 A Plant Biologist's Toolbox to Study Translation.
A Plant Biologist’s Toolbox to Study Translation.

Improve communication for interdisciplinary team work on the storage of digital information in DNA.

Close collaboration between specialists from different backgrounds and working in different scientific domains is an effective strategy to address the challenges in the area of ​​the interface between biology, chemistry, physics and engineering. Communication in collaboration alone can be challenging. Even when the project is successfully completed, so the publication – of multi-wrote – has the potential to falter.

Some, both in the field and outside, may be able to fully understand the work as a whole. This needs addressed in order to facilitate efficient work, peer review, accessibility and impact to a greater audience. We are an interdisciplinary team working in the nascent scientific field, which repurposing DNA as digital information storage media. In this note, we highlight some of the difficulties that arise from the collaboration and outline our efforts to improve communication through the glossary and controlled vocabulary and accessibility through a plain-language summary of the brief.

D(+)-Sucrose (Molecular Biology Grade)

CE225 1 kg
EUR 70.00

D(+)-Sucrose (Molecular Biology Grade)

CE226 5 kg
EUR 173.00

Urea, suitable for molecular biology

GE1210-1KG 1 kg
EUR 89.00

Urea, suitable for molecular biology

GE1210-500G 500 g
EUR 64.00

BCIP (Molecular Biology Grade)

CE108 250 mg
EUR 63.00

BCIP (Molecular Biology Grade)

CE109 1 g
EUR 90.00

CHAPS (Molecular Biology Grade)

CE114 1 g
EUR 55.00

CHAPS (Molecular Biology Grade)

CE115 5 g
EUR 131.00

CHAPS (Molecular Biology Grade)

CE116 25 g
EUR 410.00

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 60.00

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 133.00

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 319.00

Dimethylsulfoxide (Molecular Biology Grade)

CE120 100 ml
EUR 55.00

Dimethylsulfoxide (Molecular Biology Grade)

CE121 500 ml
EUR 92.00

DTT (Molecular Biology Grade)

CE131 5 g
EUR 78.00

DTT (Molecular Biology Grade)

CE132 10 g
EUR 111.00

DTT (Molecular Biology Grade)

CE133 25 g
EUR 203.00

Glycine (Molecular Biology Grade)

CE158 1 kg
EUR 70.00

Glycine (Molecular Biology Grade)

CE159 5 kg
EUR 190.00

HEPES (Molecular Biology Grade)

CE171 100 g
EUR 82.00

HEPES (Molecular Biology Grade)

CE172 500 g
EUR 224.00

HEPES (Molecular Biology Grade)

CE173 1 kg
EUR 354.00

Lysozyme (Molecular Biology Grade)

CE188 1 g
EUR 59.00

Lysozyme (Molecular Biology Grade)

CE189 10 g
EUR 206.00

NAD (Molecular Biology Grade)

CE196 1 g
EUR 60.00

NAD (Molecular Biology Grade)

CE197 5 g
EUR 138.00

NBT (Molecular Biology Grade)

CE209 1 g
EUR 103.00

NBT (Molecular Biology Grade)

CE210 5 g
EUR 300.00

Tris (Molecular Biology Grade)

CE237 500 g
EUR 89.00

Tris (Molecular Biology Grade)

CE238 1 kg
EUR 128.00

Tris (Molecular Biology Grade)

CE239 5 kg
EUR 446.00

Tween20 (Molecular Biology Grade)

CE242 1 l
EUR 89.00

Water (Molecular Biology Grade)

CE243 500 ml
EUR 52.00

Water (Molecular Biology Grade)

CE244 1 l
EUR 56.00

Ammonium sulfate (Molecular Biology Grade)

CE105 250 g
EUR 46.00

Ammonium sulfate (Molecular Biology Grade)

CE106 1 kg
EUR 60.00

Ammonium sulfate (Molecular Biology Grade)

CE107 5 kg
EUR 128.00

Bis-Acrylamid (Molecular Biology Grade)

CE110 50 g
EUR 79.00

Bis-Acrylamid (Molecular Biology Grade)

CE111 250 g
EUR 216.00

Formamide deionized (Molecular Biology Grade)

CE145 500 ml
EUR 73.00

Formamide deionized (Molecular Biology Grade)

CE146 1 l
EUR 100.00

Glycerol 87 % (Molecular Biology Grade)

CE154 1 l
EUR 78.00

Glycerol waterfree (Molecular Biology Grade)

CE155 500 ml
EUR 65.00

Glycerol waterfree (Molecular Biology Grade)

CE156 1 l
EUR 85.00

Glycerol waterfree (Molecular Biology Grade)

CE157 2.5 l
EUR 142.00

Guanidine - Hydrochloride (Molecular Biology Grade)

CE160 100 g
EUR 78.00

Guanidine - Hydrochloride (Molecular Biology Grade)

CE161 250 g
EUR 128.00

Guanidine - Hydrochloride (Molecular Biology Grade)

CE162 500 g
EUR 194.00

Guanidine - Hydrochloride (Molecular Biology Grade)

CE163 1 kg
EUR 294.00

Guanidine Thiocyanate (Molecular Biology Grade)

CE164 100 g
EUR 72.00

Guanidine Thiocyanate (Molecular Biology Grade)

CE165 500 g
EUR 160.00

Guanidine Thiocyanate (Molecular Biology Grade)

CE166 1 kg
EUR 256.00

Urea Crystalline (Molecular Biology Grade)

CE167 1 kg
EUR 60.00

Urea Crystalline (Molecular Biology Grade)

CE168 5 kg
EUR 151.00

MOPS buffer (Molecular Biology Grade)

CE194 100 g
EUR 85.00

MOPS buffer (Molecular Biology Grade)

CE195 250 g
EUR 141.00

Sodium chloride (Molecular Biology Grade)

CE205 500 g
EUR 52.00

Sodium chloride (Molecular Biology Grade)

CE206 1 kg
EUR 59.00

Sodium chloride (Molecular Biology Grade)

CE207 5 kg
EUR 103.00

Tris - Hydrochloride (Molecular Biology Grade)

CE234 250 g
EUR 83.00

Tris - Hydrochloride (Molecular Biology Grade)

CE235 500 g
EUR 120.00

Tris - Hydrochloride (Molecular Biology Grade)

CE236 1 kg
EUR 186.00

TritonX-100 (Molecular Biology Grade)

CE240 500 ml
EUR 56.00

TritonX-100 (Molecular Biology Grade)

CE241 1 l
EUR 66.00

Water, Ultrapure Molecular Biology Grade

41024-4L 4L
EUR 121.00
Description: Minimum order quantity: 1 unit of 4L

Tween 20, Molecular Biology Grade

T9100-010 100ml
EUR 72.00

Tween 20, Molecular Biology Grade

T9100-050 500ml
EUR 111.00

Tween 20, Molecular Biology Grade

T9100-100 1L
EUR 134.00

Phenol, (Carbolic acid) Double distilled for Molecular Biology

PD0252 500g
EUR 160.49
  • Product category: Biochemicals/Misc. Biochemicals

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 181.00

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1L 1 l
EUR 77.00

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE135 250 g
EUR 60.00

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE136 500 g
EUR 72.00

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE137 1 kg
EUR 104.00

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE138 5 kg
EUR 349.00

D(+)-Glucose waterfree (Molecular Biology Grade)

CE148 500 g
EUR 56.00

D(+)-Glucose waterfree (Molecular Biology Grade)

CE149 1 kg
EUR 63.00

D(+)-Glucose waterfree (Molecular Biology Grade)

CE150 5 kg
EUR 150.00

Yeast extract powder (Molecular Biology Grade)

CE169 500 g
EUR 111.00

Hyaluronidase Grade I (Molecular Biology Grade)

CE174 1 g
EUR 194.00

Hyaluronidase Grade I (Molecular Biology Grade)

CE175 5 g
EUR 767.00

Magnesium acetate - Tetrahydrate (Molecular Biology Grade)

CE190 500 g
EUR 82.00

NADH - Disodium salt (Molecular Biology Grade)

CE198 1 g
EUR 76.00

NADH - Disodium salt (Molecular Biology Grade)

CE199 5 g
EUR 204.00

NADP - sodium salt (Molecular Biology Grade)

CE200 250 mg
EUR 77.00

NADP - sodium salt (Molecular Biology Grade)

CE201 1 g
EUR 159.00

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE202 25 mg
EUR 59.00

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE203 100 mg
EUR 105.00

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE204 500 mg
EUR 312.00

SSC Buffer (20X) (Molecular Biology Grade)

CE229 1 l
EUR 72.00

XTT sodium salt (Molecular Biology Grade)

CE250 100 mg
EUR 174.00

XTT sodium salt (Molecular Biology Grade)

CE251 500 mg
EUR 510.00

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE100 50 g
EUR 107.00

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE101 100 g
EUR 161.00

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE102 250 g
EUR 323.00

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE103 500 g
EUR 547.00

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE104 1 kg
EUR 969.00

EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade)

CE141 500 ml
EUR 73.00

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE178 500 g
EUR 91.00

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE179 2.5 kg
EUR 251.00

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE180 500 g
EUR 87.00

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE181 2.5 kg
EUR 246.00

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE182 500 g
EUR 90.00

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE183 2.5 kg
EUR 251.00

LB-Medium - Powder according to Miller (Molecular Biology Grade)

CE184 2.5 kg
EUR 246.00

Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g

41028-100G 100G
EUR 222.00
Description: Minimum order quantity: 1 unit of 100G

Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g

41028-25G 25G
EUR 109.00
Description: Minimum order quantity: 1 unit of 25G

Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g

41028-500G 500G
EUR 719.00
Description: Minimum order quantity: 1 unit of 500G

Sucrose

abx082163-500g 500 g
EUR 175.00
  • Shipped within 5-10 working days.

Sucrose

abx082558-500g 500 g
EUR 189.00
  • Shipped within 5-10 working days.

Sucrose

GC4473-100G 100 g
EUR 38.00

Sucrose

GC4473-10KG 10 kg
EUR 181.00

Sucrose

GC4473-1KG 1 kg
EUR 54.00

Sucrose

GC4473-250G 250 g
EUR 40.00

Sucrose

GC4473-2KG 2 kg
EUR 73.00

Sucrose

GC4473-500G 500 g
EUR 44.00

Sucrose

GC4473-5KG 5 kg
EUR 118.00

Sucrose

HY-B1779 10mM/1mL
EUR 113.00

Sucrose

TB01275 8XX100mg
EUR 274.00

SUCROSE

S19-117-10kg 10 kg
EUR 540.00

SUCROSE

S19-117-2kg 2kg
EUR 157.00

SUCROSE

S19-117-500g 500 g
EUR 79.00

Sucrose

SB0498 500g
EUR 59.54
  • Product category: Biochemicals/Carbohydrates/Sugars

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 168.00
Description: Bioscience Mol Bio; Agarose

Sucrose (saccharose)

16-0020-1000 1 kg
EUR 93.00

Iron sucrose

GC0709-100G 100 g
EUR 190.00

Iron sucrose

GC0709-25G 25 g
EUR 86.00

Iron sucrose

GC0709-50G 50 g
EUR 122.00

Sucrose stearate

GC2889-25G 25 g
EUR 82.00

Sucrose stearate

GC2889-5G 5 g
EUR 45.00

Sucrose benzoate

GC4185-1KG 1 kg
EUR 142.00

Sucrose benzoate

GC4185-500G 500 g
EUR 94.00

Sucrose octaacetate

GC6190-1KG 1 kg
EUR 122.00

Sucrose octaacetate

GC6190-250G 250 g
EUR 59.00

Sucrose octaacetate

GC6190-500G 500 g
EUR 82.00

Sucrose Assay Kit

55R-1468 100 assays
EUR 689.00
Description: Assay Kit for detection of Sucrose in the research laboratory

Dodecanoyl-D-sucrose

GC7623-250MG 250 mg
EUR 102.00

3,6-Disinapoyl sucrose

TB0776 20mg
EUR 267.00

100 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS

46-000-CI 100 mL/pk
EUR 57.00
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

1 L, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS

46-000-CM 1L/pk
EUR 98.00
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

500 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS

46-000-CV 500 mL/pk
EUR 106.00
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3058.00
Description: Bioscience Mol Bio; Agarose

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2038.00
Description: Bioscience Mol Bio; Agarose

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 772.00
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 3737.00
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2075.00
Description: Bioscience Mol Bio; Agarose

Sucrose Synthase (SUS1) Antibody

20-abx319796
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Sucrose (Plant) Assay Kit

abx298802-100Assays 100 Assays
EUR 472.00
  • Shipped within 5-10 working days.

Sucrose Synthase Assay Kit

abx298804-100Assays 100 Assays
EUR 566.00
  • Shipped within 5-10 working days.

Poly(sucrose-co-epichlorhydrin)

GM4452-100G 100 g
EUR 341.00

Sucrose octasulfate sodium salt

GK4916-1G 1 g
EUR 221.00

Sucrose octasulfate sodium salt

GK4916-250MG 250 mg
EUR 102.00

Sucrose octasulfate-aluminum complex

GC0234-10G 10 g
EUR 229.00

Sucrose octasulfate-aluminum complex

GC0234-1G 1 g
EUR 70.00

Sucrose octasulfate-aluminum complex

GC0234-5G 5 g
EUR 150.00

Sucrose octasulfate potassium salt

GC1775-1G 1 g
EUR 150.00

EnzyChrom? Sucrose Assay Kit

ESUC-100 100
EUR 344.93
Description: Quantitative determination of sucrose by colorimetric (565nm) method. Procedure: 70 min. Kit size: 100 tests. Detection limit: 17 µM. Shelf life: 3 months. Shipping: on ice; storage: -20°C.

PHENOL RED SUCROSE BROTH

P16-123-10kg 10 kg
EUR 835.00

PHENOL RED SUCROSE BROTH

P16-123-2Kg 2 Kg
EUR 221.00

PHENOL RED SUCROSE BROTH

P16-123-500g 500 g
EUR 96.00

3, 6?-Disinapoyl Sucrose

N2096-20 20 mg
EUR 502.00
Description: Extracted from Polygala tenuifolia Willd. dried roots;Store the product in sealed, cool and dry condition

Sucrose Synthase (SUS1) Antibody (HRP)

20-abx319797
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Sucrose Synthase (SUS1) Antibody (FITC)

20-abx319798
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Sucrose Synthase (SUS1) Antibody (Biotin)

20-abx319799
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Sucrose Phosphate Synthase Assay Kit

abx298805-100Assays 100 Assays
EUR 629.00
  • Shipped within 5-10 working days.

Saccharum officinarum Sucrose synthase (SUS1)

1-CSB-EP335680SVV
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 41.3 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Saccharum officinarum Sucrose synthase(SUS1),partial expressed in E.coli

Sucrose, BP, EP, USP grade

GC3219-100G 100 g
EUR 41.00

Sucrose, BP, EP, USP grade

GC3219-10KG 10 kg
EUR 229.00

Sucrose, BP, EP, USP grade

GC3219-1KG 1 kg
EUR 62.00

Sucrose, BP, EP, USP grade

GC3219-250G 250 g
EUR 45.00

Sucrose, BP, EP, USP grade

GC3219-5KG 5 kg
EUR 142.00

3-​Feruloyl-​1-​Sinapoyl sucrose

HY-N4320 10mg
EUR 574.00

We hope to stimulate discussion earlier in this emerging field of how our community might improve the description and presentation of our work to facilitate clear communication within and between research groups and accessibility increase for those who are not familiar with our field each – good the molecular biology, computer science, information theory or others that may become relevant in the future. To enable open and inclusive discussion we have made a list of controlled vocabulary terms and as a cloud-based shared document and we invite other scientists to criticisms and suggestions and contribute their own ideas.